Novel assay for the influenza virus M2 channel activity

FEBS Letters

Forgey, Robert W.¹  Rader, Randall K.¹  Marino, Margaret H.¹  Giffin, Kelleen¹ 

[1] Department of Protein Biochemistry, Searle Research and Development, St. Louis, MO, USA

关键词: Influenza A;    M2;    Whole-cell recording;    Amantadine;    Xenopus oocyte;    Natural product screening;    M2;    matrix protein 2;    RNA;    ribonucleic acid;    cRNA;    cDNA derived mRNA;    CNS;    central nervous system;    NMDA;    MES;    2-(4-morpholino)-ethane sulfonic acid;    HEPES;    N-[hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid];    sND96;    ND96 supplemented with 2.5 mM sodium pyruvate (Gibco);    100 U/ml penicillin (Gibco);    100 μg/ml streptomycin (Gibco);   

DOI  :  10.1016/0014-5793(94)01369-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The influenza A M2 ion channel was expressed and activity characterized in Xenopus oocytes. Based on the activation properties of the channels, a high throughput, non-electrophysiological screening assay was developed in order to identify novel inhibitors of the channel. This will facilitate discovery of novel agents to treat influenza viral infections.

【 授权许可】

Unknown