Retinoic acid induces BDNF responsiveness of sympathetic neurons by alteration of Trk neurotrophin receptor expression

FEBS Letters

Matsuoka, Ichiro¹  Kobayashi, Miwako¹  Kurihara, Kenzo¹ 

[1] Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060, Japan

关键词: Sympathetic neuron;    Retinoic acid;    Neurotrophin;    Neurotrophin receptor;    Reverse transcription-polymerase chain reaction;    RA;    retinoc acid;    SCG;    superior cervical ganglion;    NGF;    nerve growth factor;    BNDF;    brain-derived neurotrophic factor;    NT-3;    neurotrophin-3;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/0014-5793(94)01238-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of high affinity neurotrophin receptors (TrkA, TrkB, and TrkC) determines the survival response of different populations of neurons to specific members of the neurotrophin family, including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3). However, the mechanism which controls the expression of neurotrophin receptors during neuronal development is largely unknown. Here we show that the treatment of the cultured sympathetic neurons from newborn rat superior cervical ganglia (SCG) with retinoic acid (RA), a derivative of vitamin A, suppressed the expression of trkA mRNA and induced the expression of trkB mRNA. Expression of the functional TrkB receptor was confirmed by the emergence of trophic dependence of these neurons on BDNF in the absence of NGF. Differential regulation of trk mRNAs by RA provides a possible model for the establishment of neurotrophin dependence of peripheral neurons.

【 授权许可】

Unknown