Synaptotagmin I is essential for Ca2+‐independent release of neurotransmitter induced by α‐latrotoxin

FEBS Letters

Takahashi, Masami²  Ogura, Akihiko²  Shoji-Kasai, Yoko²  Grasso, Alfonso¹  Yoshida, Akira²  Kuwahara, Reiko² 

[1] Institute of Cell Biology, CNR, viale Marx 43, 00137 Roma, Italy;Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194, Japan

关键词: Neurotransmitter release;    α-Latrotoxin;    Neurexin;    Synaptotagmin;    Exocytosis;    Synaptic transmission;   

DOI  :  10.1016/0014-5793(94)01069-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α-Latrotoxin causes massive release of norepinephrine from clonal rat pheochromocytoma PC12 cells, in the absence of external Ca²⁺, by an unknown mechanism. The effect almost disappeared in PC12 variant cells deficient in synaptotagmin I, a synaptic vesicle protein, and was rescued by transfecting the synaptotagmin I gene. These results indicate that synaptotagmin I is essential for the Ca²⁺-independent action of α-latrotoxin in PC12 cells.

【 授权许可】

Unknown