On the structure of the ‘synaptosecretosome’ Evidence for a neurexin/synaptotagmin/syntaxin/Ca2+ channel complex

FEBS Letters

Shamotienko, Oleg²  Grishin, Eugene¹  Betz, Heinrich²  O'Connor, Vincent M.² 

[1] M.M. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117871, GSP-7 Moscow, V-437, Miklukho-Maklaya, 16170, Russia;Abteilung Neurochemie, Max-Planck-Institut für Hirnforschung, Deutschordenstrasse 46, 6000 Frankfurt am Main 71, Germany

关键词: α-Latrotoxin;    Neurexin;    Synaptotagmin;    Syntaxin;    Ca2− channel;    Synaptosecretosome;    ω-Ctx;    ω-conotoxin;    α-Ltx;    α-latrotoxin;    NSF;    N-ethylmaleimide-sensitive fusion protein;    PMSF;    phenylmethylsulphonyl fluoride;    SDS-PAGE;    sodium dodecylsulphate polyacrylamide gel electrophoresis;    SNAPs;    soluble NSF attachment proteins;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/0014-5793(93)81802-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recent experiments have identified interactions between presynaptic and synaptic vesicle membrane proteins, that might be important in organizing the components of the fast neurotransmitter release mechanism to ensure that the process follows a rapid time course. Here we extend previous investigations to show that in addition to the α-latrotoxin receptor (neurexin) and synaptotagmin another presynaptic protein, syntaxin, co-purifies on a α-latrotoxin affinity column. This implies that syntaxin is associated with these two molecules in a complex; a conclusion supported by the immunoprecipitation of [¹²⁵I]latrotoxin binding by syntaxin antibodies. In addition, antibodies against syntaxin and the α-latrotoxin receptor immunoprecipitate [¹²⁵I]ω-conotoxin binding sites, indicating that calcium channels are associated with this complex. Thus, neurexin, synaptotagmin, syntaxin, and calcium channels can be found in a structure we propose to call the ‘synaptosecretosome’. The components of the synaptosecretosome, in association with additional proteins, are postulated to organize the process of neurotransmitter release.

【 授权许可】

Unknown