A comparison and analysis of the toxicity and receptor binding properties of Bacillus thuringiensis CryIC ∂‐endotoxin on Spodoptera littoralis and Bombyx mori

FEBS Letters

Chaufaux, Josette¹  Pauron, David³  Sanchis, Vincent² 

[1] Station de Recherches de Lutte Biologique, INRA, La Minière, 78285 Guyancourt Cedex, France;Unité de Biochimie Microbienne, Institut Pasteur, 75724 Paris Cedex 15, France;Laboratoire de Biologie des Invertébrés, INRA, BP 2078, 06606 Antibes Cedex, France

关键词: ∂-Endotoxin;    Toxicity;    Binding affinity;    Bacillus thuringiensis;    Spodoptera littoralis;    Bombyx mori;   

DOI  :  10.1016/0014-5793(94)01057-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding of l-[³⁵S]methionine in vivo labelled CryIC toxin to its receptor in brush border membrane vesicle (BBMV's) prepared from Spodoptera littoralis and Bombyx mori was studied. Both insect species were highly susceptible to the CryIC toxin in bioassays, B. mori being 7-fold more sensitive to CryIC than S. littoralis (LC50's of 10 ng/cm² and 70 ng/cm², respectively). Competition and direct binding experiments revealed saturable high-affinity binding sites on BBMV's from both insects which had similar binding characteristics for the CryIC toxin (K _d = 10 nM, B _max = 8 to 9 pmol/mg BBMV's and IC₅₀ = 37 nM for both insect species). Thus a specific receptor for the CryIC toxin is present in both insect species and the 7-fold greater potency of CryIC towards B. mori is not due to qualitative or quantitative differences in binding affinity or receptor site concentration. Dissociation experiments also indicated that the binding of [³⁵S]CryIC to B. mori BBMV's is partially reversible.

【 授权许可】

Unknown