Isolation and toxicity test of Bacillus thuringiensis from Sekayu region soil, South Sumatra on Spodopteralitura

International Symposium on Food and Agro-biodiversity 2017

农业科学;生物科学

Afriani, S.R.^1 ; Pujiastuti, Y.^1 ; Irsan, C.^1 ; Damiri, N.^1 ; Nugraha, S.^2 ; Sembiring, E.R.^2

Study Program of Crop Science, Post Graduate Program, Sriwijaya University, South Sumatra, Palembang, Indonesia^1

Research Center for Biotechnology, Indonesian Institute of Sciences, Jakarta, Indonesia^2

关键词: Baccilus thuringiensisi;    Bacillus thuringiensis;    Bacterial isolates;    Biological controls;    Morphological observations;    Screening tests;    Spodoptera litura;    Survey methods;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/102/1/012066/pdf
DOI  :  10.1088/1755-1315/102/1/012066

来源: IOP

【 摘 要 】

This study aimed to obtain bacterial isolates B. thuringiensis potential as a biological control against pests Spodoptera litura. The research was conducted at the Laboratory of Pest and Disease Department, Agricultural Faculty of Sriwijaya University, Campus Inderalaya Ogan Ilir, South Sumatera, from March to June 2017. The study was conducted with survey method and laboratory trial. The results showed that of the 50 soil samples from three villages selected through morphological observation, reaction staining, KOH test, catalase test, producing 13 bacterial isolates. Screening of the 13th toxicity of the isolates suspected B. thuringiensis against S. litura larvae was investigated. Based on the toxicity screening test the following facts were obtained: five isolates ie KJ2M2, KJ3E1, KJ3JB1, KJ3D3and KJ3D5were lower toxicity than Dipel, two isolates ie KJ3K4and KJ3D3which had the same toxicity to Dipel. Five isolates the KJ3E3, KJ3BW5, KJ3JB5, KJ3D1and LC2, LC3known to have effectiveness until the seventh day reached 40%. There was one isolate that is KJ3BW5which was more effective compared to Dipel as comparison.