Nitric oxide and proteoglycan biosynthesis by human articular chondrocytes in alginate culture

FEBS Letters

Stefanovic-Racic, M.¹  Häuselmann, H.J.²  Evans, C.H.¹  Michel, B.A.²  Oppliger, L.³ 

[1] Ferguson Laboratory, Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 986 Scaife Hall, Pittsburgh, PA 15261, USA;Department of Rheumatology, University Hospital, Gloriastrasse 25, 8091 Zürich, Switzerland;M.E. Müller Institute for Biomechanics, University of Bern, Murtenstrasse 35, PO Box 30, 3010 Bern, Switzerland

关键词: Nitric oxide;    Interleukin-1;    Chondrocyte;    Proteoglycan synthesis;    Arthritis;    Human articular cartilage;    hrIL-1;    human;    recombinant interleukin-1;    hrIL-1ra;    human;    recombinant interleukin-1 receptor antagonist;    NMA;    N G-monomethyl-l-arginine;    NO;    nitric oxide;    NOS;    nitric oxide synthase;    PG;    proteoglycan;    SNAP;    S-nitrosyl-acetyl-d;    l-penicillamine;   

DOI  :  10.1016/0014-5793(94)00994-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Interleukin-1α and β induced the production of large amounts of nitric oxide by normal, human articular chondrocytes in alginate culture; at the same time the biosynthesis of proteoglycan was strongly suppressed. In a dose-dependent manner, N ^G-monomethyl-l-arginine both inhibited nitric oxide formation and relieved the suppression of proteoglycan synthesis. However concentrations of N ^G-monomethyl-l-arginine which completely prevented nitric oxide production only partially restored proteoglycan biosynthesis, even at low doses of interleukin-1 where suppression of proteoglycan synthesis was modest. The organic donor of nitric oxide, S-nitrosyl-acetyl-d,l- penicillamine also inhibited proteoglycan biosynthesis, but not as extensively as interleukin-1. These data suggest that interleukin-1 suppresses synthesis of the cartilaginous matrix through more than one mechanism, at least one of which is dependent upon the production of nitric oxide.

【 授权许可】

Unknown