Effects of dideoxyforskolin on proteoglycan synthesis and structure in embryonic chick chondrocyte cultures

FEBS Letters

Peng, Chun-Fu¹  Smith, W.Grady¹  Elders, M.Joycelyn¹  Hu, Lie-Min¹  Kemp, Stephen F.¹ 

[1] Departments of Biochemistry and Molecular Biology and Pediatrics, University of Arkansas for Medical Sciences, 4301 W. Markham, Little Rock, AR 72205, USA

关键词: Forskolin;    Dideoxyforskolin;    Cyclic adenosine monophosphate;    Chondrocyte;    Proteoglycan synthesis;   

DOI  :  10.1016/0014-5793(90)81243-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

1,9-Dideoxyforskolin inhibits proteoglycan synthesis and xyloside-initiated glycosaminoglycan (GAG) synthesis in chick embryo chondrocytes. Dideoxyforskolin does not affect the length of xyloside-initiated GAG chains secreted into the medium but chains from the dense proteoglycan secreted into the medium appear slightly longer. Incorporation of labeled serine into the dense proteoglycan and subsequent digestion with Pronase revealed a dramatic decrease in percent of total radioactivity associated with GAG chains in the proteoglyean from cultures treated with forskolin or dideoxyforskolin. These observations suggest that these diterpenes have a specific inhibitory effect on chain initiation reactions and thus may be useful tools in the study of proteoglycan synthesis and processing.

【 授权许可】

Unknown