Multi‐step processing of procathepsin L in vitro

FEBS Letters

Kominami, Eiki¹  Ishidoh, Kazumi¹ 

[1] Department of Biochemistry, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Proteolytic processing;    Procathepsin L;    Cathepsin L;    Proteinase activity;    SDS-PAGE;    polyacrylamide gel electrophoresis in the presence of sodium dodesyl sulfate;    Z-FR-MCA;    carbobenzoxy-l- phenylalanyl-l-arginine 4-methyl-coumaryl-7-amide;    AMC;    7-amino-4-methyl-coumarin;    SDS;    sodium dodesyl sulfate;   

DOI  :  10.1016/0014-5793(94)00924-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The proteolytic processes involved in the conversion of procathepsin L to cathepsin L on a negatively charged surface, dextran sulfate, were studied. Upon incubation for 30 min at 37°C, pH 5.5 with dextran-sulfate and dithiothreitol, purified procathepsin L showed maximal activation and, correspondingly, the complete conversion to the 30 kDa, single chain mature form of enzyme was observed. In contrast, incubation under the same conditions on ice rather than at 37°C for 30 or 60 min resulted in partial proteolysis to produce a 31 kDa form without a significant increase in activity. Amino terminal amino acid sequence analyses showed that the 30 kDa form obtained by incubation at 37°C corresponds to the purified form of mature cathepsin L with a 2 amino acid extension at the amino terminal, and that the 31 kDa form generated by incubation on ice possesses a 6 amino acid amino terminal extension, suggesting that the activation and processing of procathepsin L are different processes, and that 4 amino acid residues (Glu-Pro-Leu-Met) at the carboxyterminal in the propeptide function to prevent the activation of processed cathepsin L.

【 授权许可】

Unknown