The mechanisms and regulation of procathepsin L secretion from osteoclasts in bone resorption

FEBS Letters

Kakegawa, Hisao¹  Kamioka, Hiroshi²  Katunuma, Nobuhiko¹  Lenar≷cic̆, Brigita³  Sumitani, Koji²  Kawata, Terushige²  Tagami, Kahori²  Turk, Vito³ 

[1]Institute for Health Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770, Japan

[2]Department of Orthodontics, School of Dentistry, The University of Tokushima, Kuramoto-cho, Tokushima 770, Japan

[3]Department of Biochemistry, J. Stefan Institute, Jamova, 39, 61000 Ljubljana, Slovenia

关键词: Procathepsin L;    Cysteine proteinase;    Parathyroid hormone;    Calcitonin;    Monensin;    Bone resorption;    Z;    benzyloxycarbonyl;    MCA;    methylcoumarylamide;    E-64-a;    N-(l-3-trans-carboxyoxirane-2-carbonyl)-l-leucine-4-aminobu-tyl-amine;    CA-074;    N-(l-3-trans-proryl-carbamoloxirane-2-2-carbonyl)-l-isoleucyl-l-proline;   

DOI  :  10.1016/0014-5793(94)80522-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppressed by the addition ofcalcitonin. The Z-Phe-Arg-MCA hydrolysing activity increased in the medium through the effect of PTH is considered to be a kind of procathepsin L by Western blotting analysis, and was suppressed by calcitonin addition. Furthermore, monensin inhibited not only the PTH-induced pit formation, but also cysteine proteinase activity in osteoclasts. Therefore, the procathepsin L excreted might be transferred from endothelial reticulum via Golgi and/or via lysosomes.

【 授权许可】

Unknown