Procathepsin L‐specific antibodies that recognize procathepsin L but not cathepsin L

FEBS Letters

Takeda-Ezaki, Mitsue¹  Kominami, Eiki¹  Ishidoh, Kazumi¹ 

[1] Department of Biochemistry, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Procathepsin L;    Cathepsin L;    Procathepsin L-specific antibody;    Con A;    concanavalin A;    CNBr;    cyanogen bromide;    DMEM;    Dulbecco's modified Eagle's minimum essential medium;    ELISA;    enzyme-linked immunosorbent assay(s);    PBS(−);    Dulbecco's phosphate buffered saline;    Ras-NIH;    NIH3T3 cells transformed by v-Ha-ras;    SDS-PAGE;    sodium dodecylsulfate polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(93)81116-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Procathepsin L was purified to apparent homogeneity from the culture medium of v-Ha-ras transformed NIH3T3 (Ras-NIH) cells in three steps; anion-exchange chromatography, gel nitration, and re-gel filtration. SDS-PAGE analyses revealed that the purified samples contained only the precursor form, procathepsin L, but not the mature enzyme, cathepsin L. Antibodies against purified procathepsin L were raised. These recognized both rat cathepsin L and the purified procathepsin L. To isolate procathepsin L-specific antibodies that did not recognize cathepsin L, sequential affinity chromatography procedures were carried out. Immunoblot analyses showed that the procathepsin L-specific antibodies recognized only procathepsin L, but not cathepsin L.

【 授权许可】

Unknown