The regulation of sodium‐dependent transport of anionic amino acids in cultured human fibroblasts

FEBS Letters

Visigalli, Rossana¹  Gazzola, Gian C.¹  Bussolati, Ovidio¹  Franchi-Gazzola, Renata¹ 

[1] Istituto di Patologia Generale, Università degli Studi di Parma, Via Gramsci 14, 43100 Parma, Italy

关键词: Growth factor;    Proliferation;    PKC;    l-Glutamate;    l-Aspartate;    Human fibroblast;    BK;    bradykinin;    8-Br-cAMP;    8-Bromo-adenosine-3′:5′-monophosphate (cyclic);    DiC8;    1;    2-dioctanoyl-sn-glycerol;    DiC14;    1;    2-dimyristoyl-sn-glycerol;    DMEM;    Dulbecco's modified Eagle's medium;    FBS;    fetal bovine serum;    PDBu;    phorbol 12;    13-dibutyrate;    4α-PDD;    4α-phorbol 12;    13-didecanote;    PDGF;    platelet-derived growth factor;    PKA;    protein kinase A;    PKC;    protein kinase C;    PMA;    phorbol 12-myristate 13-acetate;    PLC-γ;    phospholipase C-γ;   

DOI  :  10.1016/0014-5793(94)00929-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In cultured human fibroblasts the transport of anionic amino acids through the sodium-dependent system X⁻ _AG is stimulated rapidly and transiently by phorbol 12,13-dibutyrate. Transport stimulation is consistent with an effect due to the activation of protein kinase C. Bradykinin (1 μM) and PDGF-AA (100 ng/ml) also stimulate the activity of system X⁻ _AG. The bradykinin effect appears to be fully dependent upon PKC activation whereas the stimulation of aspartate transport by PDGF-AA is also due to PKC-independent mechanisms.

【 授权许可】

Unknown