Growth factors stimulate phosphorylation of CTP:Phosphocholine cytidylyltransferase in HeLa cells

FEBS Letters

Orfanos, Constantin E.²  Geilen, Christoph C.²  Wieder, Thomas²  Wieprecht, Marcus¹ 

[1]Institute of Molecular Biology and Biochemistry, University Medical Center Benjamin Franklin, Free University of Berlin, Berlin, Germany

[2]Department of Dermatology, University Medical Center Benjamin Franklin, Free University of Berlin, Berlin, Germany

关键词: CTP:phosphocholine cytidylyltransferase;    Growth factor;    MAP kinase;    BCA;    bicinchoninic acid;    ECL;    enhanced chemiluminescence;    EGF;    epidermal growth factor;    DMEM;    Dulbecco's modified Eagle's medium;    CT;    cytidylyltransferase;    MAP kinase;    mitogen-activated protein kinase;    MOPS;    4-morpholinopropanesulfonic acid;    PC;    phosphatidylcholine;    PKA;    cAMP-dependent protein kinase;    PKC;    protein kinase C;    BSA;    bovine serum albumin;    PBS;    phosphate-buffered saline;    EDTA;    ethylenediamine tetraacetic acid;    EGTA;    [ethylene-bis(oxyethylenenitrilo)] tetraacid;    DTT;    dithiothreitol;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    p42mapk/p44mapk;    p42/p44 isoforms of mitogen-activated protein kinase;   

DOI  :  10.1016/0014-5793(94)01040-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of insulin and epidermal growth factor on the phosphorylation of CTP:phosphocholine cytidylyltransferase (EC 2.7.7.15) was investigated in HeLa cells. For the first time, cytidylyltransferase phosphorylation was shown to be influenced by growth factors in cell culture experiments. The rephosphorylation of cytidylyltransferase after an oleate-mediated dephosphorylation and translocation to membranes was increased after 2 min in the presence of insulin or epidermal growth factor by 99% and 76%, respectively, compared with controls. However, the increased phosphorylation of cytidylyltransferase did not have an effect on its subcellular distribution. Furthermore, purified cytidylyltransferase preincubated with alkaline phosphatase is a substrate for p44^mapk, a member of the mitogen-activated protein (MAP) kinase family downstream of the growth factor receptors, in vitro. In accordance with the in vivo data, in vitro phosphorylation of cytidylyltransferase by p44^mapk occurred after 2 min.

【 授权许可】

Unknown