Mechanical stress induces DNA synthesis in PDL fibroblasts by a mechanism unrelated to autocrine growth factor action

FEBS Letters

Basdra, Efthimia K¹  Papavassiliou, Athanasios G²  Kletsas, Dimitris³ 

[1] Poliklinik für Kieferorthopädie, Universität Heidelberg, INF 400, D-69120 Heidelberg, Germany;Department of Biochemistry, School of Medicine, University of Patras, GR-26110 Patras, Greece;Institute of Biology, N.C.S.R. `Demokritos', GR-15310 Athens, Greece

关键词: Periodontal ligament fibroblast;    Mechanical stretch;    Mechanotransduction;    DNA synthesis;    Growth factor;    Autocrine mechanism;    PDL;    periodontal ligament;    GTPase or GTP-binding protein;    guanosine triphosphate-binding protein;    PDGF;    platelet-derived growth factor;    TGF-β;    transforming growth factor-beta;    DMEM;    Dulbecco's modified Eagle's medium;    FCS;    fetal calf serum;    PBS;    phosphate-buffered saline;    SDS;    sodium dodecyl sulfate;    PKA;    protein kinase A;    PKC;    protein kinase C;    bFGF;    basic fibroblast growth factor;   

DOI  :  10.1016/S0014-5793(98)00695-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Periodontal regeneration is thought to require the proliferation of stress-sensitive periodontal ligament (PDL) fibroblast cells. The influence of physiological amounts of mechanical stretching on the DNA synthesis potential of human PDL fibroblasts was examined by means of an established, simple in vitro system of stretch application. A significant increase in the relative levels of incorporation of tritiated thymidine was observed in cultures stretched for 1–6 h. Neutralising antibodies for platelet-derived growth factor (PDGF) and transforming growth factor-beta (TGF-β) did not blunt the DNA synthesis induction. This mitogenic response to stretch appears to be independent of an autocrine mechanism involving growth factors in general, because stretch-conditioned medium, when transferred to non-stretched fibroblasts, did not mimic the mitogenic effect of stretch.

【 授权许可】

Unknown