Effects of mechanical strain on periodontal ligament fibroblasts in presence of Aggregatibacter actinomycetemcomitans lysate

BMC Oral Health

Agnes Schröder¹  Peter Proff¹  Eva Paddenberg¹  Christian Kirschneck¹  Julia Stumpf¹  James Deschner²  Josef Köstler³  Patrick Neubert³  Valentin Schatz³  Jonathan Jantsch³ 

[1] Department of Orthodontics, University Hospital Regensburg, Regensburg, Germany;Department of Periodontology and Operative Medicine, University Medicine Mainz, 55131, Mainz, Germany;Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany;

关键词: Periodontal ligament fibroblast;    PDL;    Orthodontic tooth movement;    Periodontitis;    Inflammation;   

DOI  :  10.1186/s12903-021-01761-3

来源: Springer

【 摘 要 】

PurposeMany adult orthodontic patients suffer from periodontitis, which is caused by oral pathogens such as the gram-negative Aggregatibacter actinomycetemcomitans (Agac). Like orthodontic tooth movement, periodontitis is associated with inflammation and alveolar bone remodelling thereby affecting orthodontic treatment. Interactions of both processes, however, are not sufficiently explored, particularly with regard to oxidative stress.MethodsAfter preincubation with Agac lysate for 24 h periodontal ligament fibroblasts (PDLF) were either stretched or compressed for further 48 h simulating orthodontic forces in vitro. We analysed the expression of genes and proteins involved in the formation of reactive oxygen species (NOX-4, ROS) and nitric oxide (NOS-2), inflammation (TNF, IL-6, PTGS-2) and bone remodelling (OPG, RANKL).ResultsAgac lysate elevated the expression of NOX-4, NOS-2, inflammatory IL-6 and PTGS-2 and the bone-remodelling RANKL/OPG ratio during compressive, but not tensile mechanical strain. Agac lysate stimulated pressure-induced inflammatory signalling, whereas surprisingly ROS formation was reduced. Pressure-induced downregulation of OPG expression was inhibited by Agac lysate.ConclusionsAgac lysate impact on the expression of genes and proteins involved in inflammation and bone remodelling as well as ROS formation, when PDLF were subjected to mechanical forces occurring during orthodontic tooth movement.

【 授权许可】

CC BY