Tyrosine‐rich acidic matrix protein (TRAMP) is a tyrosine‐sulphated and widely distributed protein of the extracellular matrix

FEBS Letters

Cronshaw, Andrew D.¹  MacBeath, Jonathan R.E.¹  Hulmes, David J.S.¹  Forbes, Euan G.¹ 

[1] Department of Biochemistry, University of Edinburgh, Hugh Robson Buiding, George Square, Edinburgh, EH8 9XD, UK

关键词: Extracellular matrix;    Tyrosine sulphation;    TRAMP;   

DOI  :  10.1016/0014-5793(94)00907-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tyrosine-rich acidic matrix protein (TRAMP; 22 kDa extracellular matrix protein; dermatopontin) is a protein that co-purifies with lysyl oxidase and with dermatan sulphate proteoglycans, with possible functions in cell—matrix interactions and matrix assembly. Using a rabbit polyclonal antiserum raised against porcine TRAMP, which cross-reacts with both the human and murine forms of the protein, we show by immunoblotting that TRAMP has a widespread tissue distribution, including skin, skeletal muscle, heart, lung, kidney, cartilage and bone. In cultures of human skin fibroblasts, TRAMP incorporates both [³⁵S]sulphate and [³H]tyrosine and is secreted into the medium, as shown by immunoprecipitation. Amino acid analysis of immunoprecipitated TRAMP demonstrates that many of the tyrosine residues in TRAMP are sulphated.

【 授权许可】

Unknown