Predominant role of the substituents on the hydroxyl groups of 3‐hydroxy fatty acids of non‐reducing glucosamine in lipid A for the endotoxic and antagonistic activity

FEBS Letters

Tanamoto, Ken-ichi¹ 

[1] National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya, Tokyo 158, Japan

关键词: Endotoxin;    Synthetic lipid A;    Lipid A precursor;    Non-toxic lipid A;    Endotoxin antagonist;   

DOI  :  10.1016/0014-5793(94)00857-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The synthetic disaccharide precursor of lipid A (406: identical to lipid IV_A) was found to reduce its endotoxic activity in mice by an order of 10⁵ or more, by replacing the hydroxyl groups with succinyl or acetyl residues. Both the succinylated and acetylated 406 were also found to antagonize the endotoxic mitogenicity on murine splenocytes. Previous studies demonstrated that the succinylated or acetylated synthetic complete lipid A preparations retained the whole endotoxic activity [1994, Infect. Immunol. 62, 1705]. The drastic contrast in all of these results suggests the importance of the substituents on the hydroxyl groups of 3-hydroxy fatty acids of non-reducing glucosamine of lipid A for the activity and for transformation to the antagonistic structure.

【 授权许可】

Unknown