Enhancement of O2 − generation and tumoricidal activity of murine macrophages by a monosaccharide precursor of Escherichia coli lipid A

FEBS Letters

Akamatsu, Yuzuru²  Nishijima, Masahiro²  Amano, Fumio²  Akagawa, Kiyoko¹ 

[1] Department of Cellular Immunology, National Institute of Health, 10-35 Kamioosaki 2-Chome, Shinagawa-ku, Tokyo 141, Japan;Department of Chemistry, National Institute of Health, 10-35 Kamioosaki 2-Chome, Shinagawa-ku, Tokyo 141, Japan

关键词: Lipid A precursor;    tMicrophage activation;    O 2 − generation;    Tumor cytotoxicity;    FBS;    fetal bovine serum;    LPS;    lipopolysaccharide;    NBT;    nitroblue tetrazolium;    PMA;    4ß-phorbol 12-myristate 13-acetate;    SOD;    Superoxide dismutase;    TNF;    tumor necrosis factor;   

DOI  :  10.1016/0014-5793(85)80121-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of a monosaccharide precursor of Escherichia coli lipid A (lipid X) on murine macrophages were studied. Lipid X is a diacylglucosamine 1-phosphate bearing β-hydroxymyristoyl groups at positions 2 and 3. Lipid X, as well as lipopolysaccharide and lipid A, enhanced O₂ ⁻ generation in mouse peritoneal macro-phages and a macrophage-like cell line, J774.1, and further induced the tumor-cytotoxic activity of peritoneal macrophages. Elimination of a 1-phosphate or 3-O-β-hydroxymyristoyl group from lipid X completely abolished these effects, suggesting that both 1-phosphate and 3-O-β-hydroxymyristoyl groups are essential for the elevated O₂ ⁻ generation and induction of turmoricidal activity due to lipid X.

【 授权许可】

Unknown