Activation of cyclic nucleotide phosphodiesterase by a monosaccharide precursor of Escherichia coli lipid A

FEBS Letters

Walters, John D.¹  Jirsa, Richard C.¹ 

[1] College of Dentistry, The Ohio State University, 305 West 12th Avenue, Columbus, OH 43210, USA

关键词: Lipopolysaccharide;    Lipid A precursor;    Cyclic-nucleotide phosphodiesterase;    cyclic AMP;   

DOI  :  10.1016/0014-5793(88)80044-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The E. coli lipid A precursor lipid X (N² ,O³ -diacylglucosamine 1-phosphate) activates calmodulin-dependent cyclic nucleotide phosphodiesterase in a noncooperative, calcium-independent manner by increasing its V _max and decreasing its K _m for substrate. The glycolipid produces half-maximal activation at 11 μg/ml and does not further enhance activation by calcium-calmodulin. Lipid X activation of phosphodiesterase requires the presence of the O ³-linked hydroxymyristoyl residue. These findings suggest that lipid X could produce some of its biological effects by modulating intracellular cAMP levels.

【 授权许可】

Unknown