Fluorescent differential display: Arbitrarily primed RT‐PCR fingerprinting on an automated DNA sequencer

FEBS Letters

Kito, Keiji¹  Ito, Takashi¹  Sakaki, Yoshiyuki¹  Mitsui, Yuko¹  Adati, Naoki¹  Hagiwara, Hisashi¹ 

[1] Human Genome Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokane-dai, Minato-ku, Tokyo 108, Japan

关键词: Different display;    Arbitrary primer;    RT-PCR;    Fingerprinting;    Flourescent DNA sequencer;   

DOI  :  10.1016/0014-5793(94)00867-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We established robust, reliable protocols for ‘Differential Display (DD),’ an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967–971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to ensure high throughput as well as improved operational safety, compared with the original one using radioactive compounds. Such ‘Fluorescent Differential Display (FDD)’ techniques will accelerate the identification of differentially expressed as well as polymorphic transcripts to address various biological questions.

【 授权许可】

Unknown