Restriction Fragment Length Polymorphism Analysis (RFLP) of Some Streptomyces Strains from Soil

Turkish Journal of Biology

EKREM ATALAN¹ 

关键词: Fingerprinting;    intergenic rDNA;    RFLP;    Streptomyces;   

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

To separate the unknown Streptomyces strains isolated from soil samples, the interspacer regions of 16S-23S rDNA of 14 isolates were amplified with PCR (polymerase chain reaction) and digested with three restriction endonucleases, namely, Bsp143I, HaeIII and MnlI. The restriction patterns were used for RFLP (restriction fragment length polymorphism) analysis. A dendrogram were constructed using the unweighed pair group method using arithmetic averages algorithm (UPGMA) after analysis restriction patterns. Five RFLP groups were obtained anone test strain was left as a single member group. RFLP profile indicated that unknown strains could be identified with data based on the interspacer region of 16S-23S DNA rapid and quickly.

【 授权许可】

Unknown