期刊论文详细信息
FEBS Letters | |
Evidence for redox forms of the Aequorea green fluorescent protein | |
Tsuji, Frederick I.1  Inouye, Satoshi1  | |
[1] Marine Biology Research Division 0202, Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA 92093, USA | |
关键词: Oxidation—reduction; Electron transfer; Protein chromophore; Energy transfer; Bioluminescence; Aequorin; | |
DOI : 10.1016/0014-5793(94)00859-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Highly purified recombinant Aequoreagreen fluorescent protein is able to undergo a reversible oxidation-reduction reaction in the presence of molecular oxygen. In the oxidized form in near UV light, the protein is highly fluorescent, but when reduced with sodium dithionite, it becomes completely non-fluorescent. On exposure to molecular oxygen the reduced, non-fluorescent protein reverts to its original fluorescent state.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020299981ZK.pdf | 358KB | download |