Aequorea green fluorescent protein

FEBS Letters

Tsuji, Frederick I.¹  Inouye, Satoshi¹ 

[1] Marine Biology Research Division 0202, Scripps Institution of Oceanography, University of California at San Diego, La Jotta, CA 92093 USA

关键词: Bioluminescence;    Aequorin;    Calcium;    Chromophore;    Energy transfer;   

DOI  :  10.1016/0014-5793(94)80472-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.

【 授权许可】

Unknown