A C‐terminal proline is required for bioluminescence of the Ca2+‐binding photoprotein, aequorin

FEBS Letters

Tsuji, Frederick I.²  Inouye, Satoshi¹  Ohmiya, Yoshihiro²  Nomura, Midori² 

[1] Yokohama Research Center, Chisso Corp., Kanazawa-ku, Yokahama, Kanagawa 236, Japan;Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, Osaka 565, Japan

关键词: Aequorin;    Ca2+-binding protein;    Bioluminescence;    Carboxyl-terminus;    Proline;    Deletion mutant;   

DOI  :  10.1016/0014-5793(91)81385-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The requirement for a proline residue at the C-terminus of the Ca²⁺-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline deleted showed a small change in secondary structure. In all cases studied, the C-terminal proline was required for bioluminescence activity.

【 授权许可】

Unknown