Photochemical labeling of membrane‐associated and channel‐forming domains of proteins directed by energy transfer

FEBS Letters

Goeldner, Maurice¹  Kotzyba-Hibert, Florence¹  Klotz, Philippe¹  Alcaraz, Marie-Lyne¹  Peng, Ling¹ 

[1] Laboratoire de Chimie Bio-organique, URA 1386 CNRS, Faculté de Pharmacie, Université Louis Pasteur Strasbourg, BP24, 67401 Illkirch-Cedex, France

关键词: Energy transfer;    Photoactivatable phospholipid;    Photoactivatable permeant;    Hydrophobic labeling;    Photolabeling;   

DOI  :  10.1016/0014-5793(94)00392-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Singlet—singlet energy transfer reactions from excited tryptophan residues to photoactivatable probes possessing a suitable chromophore, generate reactive species in the vicinity of the protein, leading to its covalent labeling. This delayed labeling process can be used to map the membrane-surrounded regions of proteins with improved efficiency when it is applied with appropriate photoactivatable phospholipids. The same principle could also be applied to the labeling of channel-forming transmembrane domains of ion channels, provided that suitable photoactivatable permeant ions were available. Both applications will be discussed with regard to their potential and feasibility.

【 授权许可】

Unknown