CGRP is expressed in primary cultures of human hepatocytes and in normal liver

FEBS Letters

Pidoux, E.¹  Clement, B.²  Jullienne, A.¹  Moukhtar, M.S.¹  Bracq, S.¹ 

[1] INSERM U349, Centre Viggo Petersen, 6 rue Guy Patin, 75010 Paris, France;INSERM U49, Hôpital Pontchaillou, 35033 Rennes, France

关键词: Paracrine;    Biosynthesis;    Calcitonin gene related peptide mRNA;    Human liver;    Polymerase chain reaction;    CGRP;    calcitonin gene related peptide;    RNase A;    ribonuclease A;    PCR;    polymerase chain reaction;    BSA;    bovine serum albumin;    RIA;    radioimmunoassay;    SSC;    sodium saline citrate;   

DOI  :  10.1016/0014-5793(94)00823-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We recently reported that human liver and primary cultures of hepatocytes express calcitonin. We therefore studied the expression of calcitonin gene related peptide (CGRP), the alternative splicing product of the calcitonin gene, in hepatocytes and liver. We used polymerase chain reaction amplification with specific primers to detect the presence of CGRP I and II messengers and a specific radioimmunoassay to measure the peptide. We report here that CGRP is synthesized by primary cultures of hepatocytes and in liver. As liver also possesses specific receptors for CGRP in non-parenchymal cells, a paracrine system could be involved in liver metabolism.

【 授权许可】

Unknown