RTCA Monitors the Inhibitory Effect of SWCNTs on the Proliferation of human liver cancer HepG2 cells

2019 International Conference on Advanced Electronic Materials, Computers and Materials Engineering

无线电电子学;计算机科学;材料科学

Zhang, Boya^2 ; Yang, Lei^1 ; Wang, Hua^1 ; Liu, Ming^1 ; Zhang, Xiuxia^3 ; Liu, Meiling^3 ; Shen, Jing^3 ; Liu, Zhipeng^4 ; Zhao, Zhuo^1 ; Zhang, Yuan^1

Tianjin Customs Technical Center for Safety of Industrial Products, Tianjin

300308, China^1

Department of Cardiology, Tianjin Nankai Hospital, Tianjin

300100, China^2

College of Biotechnology, Tianjin University of Science and Technology, 300457, China^3

Jin Kang Union Biotechnology Co.Ltd, 3000000, China^4

关键词: Cell analysis;    Hep-g2 cells;    Human hepatoma cells;    Human hepatomas;    Human liver;    Inhibitory effect;    Real time;    Single-walled carbon nanotube (SWCNTs);   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/563/5/052070/pdf
DOI  :  10.1088/1757-899X/563/5/052070

来源: IOP

【 摘 要 】

To investigate the inhibition of single-walled carbon nanotube (SWCNTs) on the proliferation of human hepatoma HepG2 cells by real-time label-free dynamic cell analysis (RTCA). METHODS: The RTCA system was used to dynamically monitor the effects of different doses (0, 0.5, 1 mg/mL) of SWCNTs on the growth of human hepatoma cells HepG2. RESULTS: SWCNTs at a concentration of 1 mg/mL significantly inhibited cell growth after 48 h of incubation, which was the optimal dose and duration for inhibition of HepG2 cell growth. Conclusion: RTCA system can accurately monitor the growth of HepG2 cells treated with SWCNTs.