Phase derivative microscopy of biological cells and tissues

Microscopy;Cell analysis;Coherence Imaging;Optical pathology;Tissue characterization

Kim, Tae Woo ; Popescu ; Gabriel

关键词: Microscopy;    Cell analysis;    Coherence Imaging;    Optical pathology;    Tissue characterization;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/44103/Tae%20Woo_Kim.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Phase derivative microscopy (PDM), including Laplace field microscopy (LFM) and gradient field microscopy (GFM), generates high contrast images of transparent specimens. These methods utilize spatial light modulation at the Fourier plane of a bright field microscope to optically obtain the derivatives of the phase and increase the contrast of the final image. The controllable spatial modulation pattern allows obtaining both the first-order and the second-order derivative of the phase. Compared to differential interference contrast (DIC) microscopy, which is a traditional method of imaging the derivative of the phase, PDM shows higher stability because there are no mechanically moving parts involved in the operation. More importantly, unlike DIC, PDM does not use polarizing optics and, thus, it is applicable to birefringent samples. Furthermore, because it is a single-shot technique, PDM allows acquisition speed as fast as the detector allows, and therefore, it is suitable for studying live biological samples. This thesis introduces the principle of PDM, the setup, and also applications of PDM, including high contrast imaging, live cell dynamics study, and unstained tissue biopsies.