Expression of a novel human myotonin protein kinase (MtPK) cDNA clone which encodes a protein with a thymopoietin‐like domain in COS cells

FEBS Letters

Sasagawa, Noboru³  Arahata, Kiichi¹  Ishiura, Shoichi³  Sorimachi, Hiroyuki³  Maruyama, Kei²  Suzuki, Koichi³ 

[1]National Institute of Neuroscience, NCNP, Kodaira, Tokyo 187, Japan

[2]National Institute for Physiological Sciences, Okazaki 444, Japan

[3]Institute of Molecular and Cellular Biosciences, the University of Tokyo, Tokyo 113, Japan

关键词: Myotonic dystrophy;    Protein kinase;    Thymopioetin;    cDNA clone;    Triplet repeat;   

DOI  :  10.1016/0014-5793(94)00808-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A full-length cDNA of human myotonin protein kinase (MtPK) was cloned and expressed in COS-1 cells. MtPK is recovered from the cytosolic fraction of the COS extract as a 70 kDa protein, which coincides with the size deduced from the predicted amino acid sequence. The sequence has a significant homology to thymopoietin, a peptide hormone of the thymus. Biochemical characteristics of MtPK expressed in COS-1 cells and its expression in rat tissues are investigated.

【 授权许可】

Unknown