Association of N‐ethylmaleimide‐sensitive factor with synaptic vesicles

FEBS Letters

Futai, Masamitsu¹  Moriyama, Yoshinori¹  Yamamoto, Akitsugu²  Tagaya, Mitsuo¹  Hong, Rong-Mao¹  Mori, Hiroyuki¹  Tashiro, Yutaka²  Fukui, Toshio¹ 

[1] Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567, Japan;Department of Physiology, Kansai Medical University, Moriguchi, Osaka 570, Japan

关键词: Synaptic vesicle;    Vesicular transport;    Neurotransmitter;    NSF;    N-ethylmaleimide-sensitive factor;    SNAP;    soluble NSF attachment protein;    SNARE;    SNAP receptor;    CHO;    Chinese hamster ovary;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(94)00778-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

N-Ethylmaleimide-sensitive factor (NSF) mediates docking and/or fusion of transport vesicles in the multi-pathways of vesicular transport. NSF was highly expressed in brain and adrenal gland. Immunostaining of cerebellum with an anti-NSF monoclonal antibody showed that NSF is predominantly localized in the molecular layers and the glomeruli of the granule cell layers. This distribution coincided well with that of synaptophysin, a marker protein of synaptic vesicles. Purification and immunoprecipitation revealed that NSF is associated with brain synaptic vesicles. The present results suggest that NSF is associated with synaptic vesicles without Ca²⁺ influx.

【 授权许可】

Unknown