cAMP‐dependent protein phosphorylation in mitochondria of bovine heart

FEBS Letters

Stanca, Maurizio Rosario¹  Sardanelli, Anna Maria¹  Technikova-Dobrova, Zuzana²  Papa, Sergio¹ 

[1] Institute of Medical Biochemistry and Chemistry, University of Bari, Bari, Italy;Institute of Microbiology, Czech Academy of Sciences, Praha, Czech Republic

关键词: Mitochondrion;    Protein phosphorylation;    Protein kinase;    Cyclic AMP;    Cyclic AMP-dependent protein kinase;    PMSF;    phenylmethylsulfonyl fluoride;    TMPD;    tetramethyl-p-phenylene-diamine dihydrochloride;    PAGE;    polyacrylamide gel-electrophoresis;    SDS;    sodium dodecylsulphate;    cAPK;    cAMP-dependent protein kinase;    C-cAPK;    catalytic subunit of cAPK;   

DOI  :  10.1016/0014-5793(94)00760-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A study is presented of the cAMP-dependent phosphorylation in bovine heart mitochondria of three proteins of 42, 16 and 6.5 kDa associated to the inner membrane. These proteins are also phosphorylated by the cytosolic cAMP-dependent protein kinase and by the purified catalytic subunit of this enzyme. In the cytosol, proteins of 16 and 6.5 kDa are phosphorylated by the cAMP-dependent kinase. It is possible that cytosolic and mitochondrial cAMP-dependent kinases phosphorylate the same proteins in the two compartments.

【 授权许可】

Unknown