Phosphorylation of mitochondrial membrane proteins: effect of the surface potential on monoamine oxidase

FEBS Letters

Famulski, Konrad S.¹  Nałȩcz, Maciej J.¹  Wojtczak, Lech¹ 

[1] Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Pasteura 3, 02-093 Warsaw, Poland

关键词: Protein phosphorylation;    Surface potential;    Monoamine oxidase;    Mitochondria;    Membrane protein;    Cyclic AMP;    ANS;    8-anilino-1-naphthalene sulphonate;    CCCP;    carbonyl cyanide-m-chlorophenylhydrazone;    cAMP;    cyclic 3′:5′-adenosine mononucleotide;   

DOI  :  10.1016/0014-5793(83)81129-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteins of mitochondrial membranes become phosphorylated when liver mitochondria are incubated with ATP in the presence of Mg²⁺. This is accompanied by an increase of the negative surface potential of mitochondrial membranes, as calculated from the dissociation constant of fluorescent probes, 8-anilino-1-naphthalene sulphonate and ethidium bromide, and by a decrease of the apparent K _m-value of mitochondrial monoamine oxidase. Protein phosphorylation, the increase of the negative surface potential and the decrease of apparent K _m of monoamine oxidase are greatly potentiated by cytoplasmic protein kinases in the presence of cyclic AMP.

【 授权许可】

Unknown