Phosphorylation of mitochondrial proteins in bovine heart

FEBS Letters

Sardanelli, Anna Maria¹  Technikova-Dobrova, Zuzana¹  Papa, Sergio¹ 

[1] Institute of Medical Biochemistry and Chemistry, University of Bari, Bari, Italy

关键词: Mitochondrion;    Protein phosphorylation;    Protein kinase;    Cyclic AMP;    Cyclic AMP-dependent protein kinase;    PMSF;    phenylmethylsulfonyl fluoride;    PAGE;    polyacrylamide gel electrophoresis;    bc 1;    ubiquinol-cytochrome c reductase;    SDS;    sodium dodecyl sulphate;   

DOI  :  10.1016/0014-5793(93)81109-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein phosphorylation by [γ-³²P]ATP in total extract and subfractions of bovine heart mitochondria has been studied. The results show that, in addition to pyruvate dehydrogenase, three mitochondrial proteins, with molecular weights of 44,000, 39,000 and 31,000 Da, are phosphorylated by a cAMP-independent mitochondrial protein kinase. Three other proteins associated with mitochondria, with molecular weights of 125,000, 19,000 and 6,500 Da, are phosphorylated by the cytoplasmic cAMP-dependent protein kinase (kinase A).

【 授权许可】

Unknown