Transfer RNA binding protein in the nucleus of Saccharomyces cerevisiae

FEBS Letters

Reinbolt, J.¹  Dirheimer, G.¹  Gangloff, J.¹  Wilhelm, M.L.¹  Wilhelm, F.X.¹ 

[1] Unité Propre de Recherche 9002 du Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire et Cellulaire, 15 rue René Descartes, 67084 Strasbourg, France

关键词: tRNA;    Saccharomyces cerevisiae;    Zuotin;    Nuclear protein;   

DOI  :  10.1016/0014-5793(94)00683-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A yeast nuclear protein that binds to tRNA was identified using a RNA mobility shift assay. Northwestern blotting and N-terminal sequencing experiments indicate that this tRNA-binding protein is identical to zuotin which has previously been shown to bind to Z-DNA [(1992) EMBO J. 11, 3787–37961. Labeled TRNA and poly(dG-m⁵dC) stabilized in the Z-DNA form identify the same protein on a Northwestern blot. In a gel retardation assay poly(dG-m⁵dC) in the Z-form strongly diminishes the binding of tRNA to zuotin. These studies establish that zuotin is able to bind to both tRNA and Z-DNA. Zuotin may be transiently associated with tRNA in the nucleus of yeast cells and play a role in its processing or transport to the cytoplasm.

【 授权许可】

Unknown