Genomic Exploration of the Hemiascomycetous Yeasts: 12. Kluyveromyces marxianus var. marxianus

FEBS Letters

Malpertuy, Alain¹  Dujon, Bernard¹  Artiguenave, François²  Blandin, Gaëlle¹  Wincker, Patrick²  Llorente, Bertrand¹ 

[1]Unité de Génétique Moléculaire des Levures, (URA 2171 du CNRS and UFR 927 Université Pierre et Marie Curie), Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France

[2]Génoscope-Centre National de Séquençage, 2 rue Gaston Crémieux P.O. Box 191, 91006 Evry Cedex, France

关键词: Intron;    Retrotransposon;    Genomic library;    tRNA;    Synteny;    Saccharomyces cerevisiae;   

DOI  :  10.1016/S0014-5793(00)02283-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

As part of the comparative genomics project ‘GENOLEVURES’, we studied the Kluyveromyces marxianus var. marxianus strain CBS712 using a partial random sequencing strategy. With a 0.2×genome equivalent coverage, we identified ca. 1300 novel genes encoding proteins, some containing spliceosomal introns with consensus splice sites identical to those of Saccharomyces cerevisiae, 28 tRNA genes, the whole rDNA repeat, and retrotransposons of the Ty1/2 family of S. cerevisiae with diverged Long Terminal Repeats. Functional classification of the K. marxianus genes, as well as the analysis of the paralogous gene families revealed few differences with respect to S. cerevisiae. Only 42 K. marxianus identified genes are without detectable homolog in the baker's yeast. However, we identified several genetic rearrangements between these two yeast species.

【 授权许可】

Unknown