The aminoacceptor stem of the yeast tRNALys contains determinants of mitochondrial import selectivity

FEBS Letters

Entelis, N.S²  Kazakova, H.A²  Martin, R.P¹  Tarassov, I.A¹ 

[1] UPR 9005 CNRS (Méchanismes Moléculaires de la Division Cellulaire et du Développement), 21 rue Rene Descartes, 67084 Strasbourg, France;Division of Molecular Biology, Biology Faculty, Moscow State University, Moscow 119899, Russia

关键词: tRNA;    Mitochondrial import;    Aminoacceptor stem;    Saccharomyces cerevisiae;    tRK1;    cytoplasmic tRNALys CUU;    tRK2;    cytoplasmic tRNALys SUU;    tRK3;    mitochondrial tRNALys U*UU;    KRS;    cytoplasmic lysyl-tRNA synthetase;    MSK;    mitochondrial lysyl-tRNA synthetase;    IDP;    import directing proteins;   

DOI  :  10.1016/S0014-5793(98)01653-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Yeast tRNA^Lys _CUU is nucleus-encoded and is partially imported into the mitochondria. Another lysine isoacceptor, tRNA^Lys _SUU, is also nucleus-encoded but is not imported. These two tRNAs differ in 21 bases. We have previously localised import selectivity determinants in the anticodon arm. By in vitro import of mutant transcripts and by expression of mutant tRNA genes in vivo we show here that the first base pair (1:72) and the discriminator base 73 are also relevant to import selectivity. Replacement of bases 1:72 in tRNA^Lys _SUU by those of tRNA^Lys _CUU makes it importable with a transport efficiency similar to natural.

【 授权许可】

Unknown