Recoverin mediates the calcium effect upon rhodopsin phosphorylation and cGMP hydrolysis in bovine retina rod cells

FEBS Letters

Gimelbrant, Alexandr A.¹  Senin, Ivan I.¹  Philippov, Pavel P.¹  Gorodovikova, Elena N.¹ 

[1]Department of Enzymology, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation

关键词: Photoreception;    Phosphorylation;    Rhodopsin;    Rhodopsin kinase;    Recoverin;    Bovine rod;    ROS;    rod outer segments;    Rh and Rh*;    ‘dark’ and bleached rhodopsin;    respectively;    RK;    rhodopsin kinase;    cGMP;    3′;    5′-cyclic guanosine monophosphate;    PDE;    cGMP-phosphodiesterase;   

DOI  :  10.1016/0014-5793(94)00661-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rhodopsin phosphorylation and in consequence cGMP hydrolysis in bovine rod outer segments are Ca²⁺ dependent in the presence of ATP. The level of rhodopsin phosphorylation decreases and the lifetime of active phosphodiesterase increases when the free [Ca²⁺] is raised from <1 nM to about 1 μM; in both cases the half-maximal effect was observed at 140–170 nM of free Ca²⁺. Antibodies to recoverin reverse both effects at high [Ca²⁺] but have no influence at low [Ca²⁺]. We conclude that the Ca²⁺ effects observed are mediated by recoverin which inhibits rhodopsin kinase at a high Ca²⁺ level.

【 授权许可】

Unknown