Peroxynitrite‐mediated oxidation of albumin to the protein‐thiyl free radical

FEBS Letters

Radi, Rafael¹  Gatti, Reynaldo M.²  Augusto, Ohara² 

[1] Department of Biochemistry, Facultad de Medicina, Universidad de la República, 11800 Montevideo, Uruguay;Department of Biochemistry, Instituto de Química, Universidade de São Paulo, CxP. 20780, 01498-970 São Paulo, Brazil

关键词: Peroxynitrite;    Albumin;    Thiols;    Thiyl radical;    Cysteine;    Spin-trapping;    BSA;    bovine serum albumin;    EPR;    electron paramagnetic resonance;    DMPO;    5;    5-dimethyl-1-pyrroline-N-oxide;    PBN;    α-phenyl-N-tert-butyl nitrone;   

DOI  :  10.1016/0014-5793(94)00625-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nitric oxide reacts with superoxide to produce peroxynitrite, which may be an important mediator of oxidant-induced cellular injury. Here we report that peroxynitrite is able to oxidize a protein, bovine serum albumin (BSA), to the corresponding protein-thiyl free radical as demonstrated by electron paramagnetic resonance (EPR)-spin-trapping experiments with both α-phenyl-N-tert-butyl nitrone (PBN) and 5,5-dimethyl-1 -pyrroline- N-oxide (DMPO). BSA radical adduct yields increased with pH indicating peroxynitrite anion as its main forming agent. Reaction with peroxynitrite may be another aspect of the antioxidant action of albumin in extracellular fluids.

【 授权许可】

Unknown