Photosensitized decomposition of S‐nitrosothiols and 2‐methyl‐2‐nitrosopropane Possible use for site‐directed nitric oxide production

FEBS Letters

Joseph, Joy¹  Kalyanaraman, B.¹  Singh, Ravinder Jit¹  Hogg, Neil¹ 

[1] Biophysics Research Institute, Medical College of Wisconsin 8701 Watertown Plank Rd., P.O. Box 26509, Milwaukee, WI 53226, USA

关键词: Nitric oxide;    Thiyl radical;    S-Nitrosoglutathione;    2-Methyl-2-nitrosopropane;    Rose Bengal;    Hematoporphyrin;    Aluminum phthalocyanine tetrasulphonate;   

DOI  :  10.1016/0014-5793(95)00065-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Irradiation of S-nitrosoglutathione (GSNO) with light (λ = 550 nm) resulted in the homolytic decomposition of GSNO to generate glutathionyl radical (GS ·) and nitric oxide (·NO), which were monitored by ESR spectrometry. Inclusion of Rose Bengal (RB) resulted in a 9-fold increase in the quantum yield for ·NO production and also an increase in the rate of thiyl radical formation. The bimolecular rate constant for the interaction of triplet RB with GSNO has been estimated to be approximately 1.2 × 10⁹ M⁻¹s⁻¹ by competition with oxygen. Hematoporphyrin (HP) also enhanced the rate of ·NO and tert-butyl radical. Aluminum 2-Methyl-2-nitrosopropane (MNP) decomposed on irradiation (λ = 660 nm) to form ·NO and tert-butyl radical. Aluminum phthalocyanine tetrasulphonate enhanced the rate of decomposition of MNP by 10-fold. These studies show that photosensitizers enhance the release of ·NO from donor compounds.

【 授权许可】

Unknown