An increase of the energy coupling capacity of submitochondrial particles by lanthanides

FEBS Letters

Vinogradov, A.D.¹  Gavrikova, E.V.¹  Grivennikova, V.G.¹ 

[1]Department of Biochemistry, School of Biology, Moscow State University, 119899 Moscow, Russian Federation

关键词: Lanthanide;    Energy coupling;    Submitochondrial particle;    SMP;    submitochondrial particles;    BSA;    bovine serum albumin;    DCCD;    N;    N′-dicyclohexylcarbodiimide;    F0;    membrane sector of the mitochondrial F0-F1 ATPsynthase;    Complex I;    NADH-ubiquinone oxidoreductase (EC 1.6.99.3);    CCCP;    carbonylcyanide-m-chlorophenylhydrazone;   

DOI  :  10.1016/0014-5793(94)00541-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

NADH and succinate oxidase activities of inside-out submitochondrial particles treated with excess oligomycin are inhibited by lanthanides (La³⁺ and Dy³⁺). Both inhibition by oligomycin and oligomycin plus lanthanides are completely relieved by an uncoupler. The respiratory control measured as the stimulation of NADH or succinate oxidation caused by the addition of uncoupler to the oligomycin-treated particles is thus increased in the presence of lanthanides. The coupling effect of lanthanides is completely prevented and rapidly reversed by excess EDTA. La³⁺ increases the extent of the aerobic energy-linked succinate-supported NAD⁺ reduction catalyzed by the oligomycin-treated submitochondrial particles. Lanthanides seem to be useful tools to increase the energy coupling capacity of the submitochondrial particles.

【 授权许可】

Unknown