Tissue specificity of mitochondrial F0F1‐ATPase activity of Lilium longiflorum plant

FEBS Letters

Itoh, Aya¹  Sekiya, Jiro¹ 

[1] Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kyoto 606-01, Japan

关键词: F0F1-ATPase;    Tissue specificity;    Submitochondrial particle;    Reconstitution;    Lipid composition;    Lilium longiflorum;    CL;    cardiolipin;    ELISA;    enzyme linked immunosorbant assay;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    PMSF;    phenylmethyl sulfonylfluoride;    SMP;    submitochondrial particles;    16:0;    palmitic acid;    18:0;    stearic acid;    18:1;    oleic acid;    18:2;    linoleic acid;    18:3;    linolenic acid;   

DOI  :  10.1016/0014-5793(94)01269-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A large difference was found in the activities of oligomycin-sensitive mitochondrial F₀F₁-ATPase isolated from different tissues of Lilium longiflorum plants. The enzyme activity of F₀F₁-ATPase in pollen was the highest, while that in bulbs was the lowest. When ATPases were cross-reconstituted from F₁-ATPases and F₁-depleted submitochondrial particles (SMP), ATPases reconstituted from F₁-depleted pollen SMP showed the higher activity regardless of the source of F₁-ATPase. Fatty acid compositions of phospholipids in SMP were also different between bulbs and pollen. These suggest that the F₀ portion and/or its environment are important for regulation of F₀F₁-ATPase activity in L. longiflorum plant.

【 授权许可】

Unknown