Structure of the nuclear encoded γ subunit of CF0CF1 of the diatom Odontella sinensis including its presequence

FEBS Letters

Strotmann, Heinrich¹  Pancic, Peter G.¹ 

[1]Institut für Biochemie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, 4000 Düsseldorf 1, Germany

关键词: F0F1-ATPase;    γ Subunit;    Protein import;    Presequence;    Diatom;    Odontella sinensis;    LHC;    light harvesting complex;    PAGE;    polyacrylamide gel electrophoresis;    PCR;    polymerase chain reaction;    bp;    basepairs;    SDS;    sodium dodecylsulfate;   

DOI  :  10.1016/0014-5793(93)81658-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a PCR-product as homologous probe for screening of a cDNA library of the diatom Odontella sinensis overlapping cDNA clones were obtained which showed homologies to atpC-genes of F₀F₁-ATPases from different sources. Comparison of the deduced amino acid sequence with the N-terminal sequence of the Odontella γ subunit obtained by protein sequencing, indicated that the complete 370 amino acid protein is processed to a mature protein of 315 amino acids. The 55 amino acids comprising the presequence consists of two segments, one resembling a signal sequence for cotranslational transport through ER membranes and one showing characteristics of a transit sequence for transport of proteins into chloroplasts of higher plants. This result is discussed with respect to the particular envelope structure of chromophytic plastids consisting of four membranes. The outer membrane contains ribosomes on its cytosolic surface. As in cyanobacterial γ subunits the regulatory sequence region, which is involved in thiol modulation of chloroplast ATPase of green algae and higher plants, is absent in the Odontella γ subunit.

【 授权许可】

Unknown