Post‐transcriptional repression of thymidine kinase expression during cell cycle and growth stimulation

FEBS Letters

Müllner, Ernst W.¹  Mikulits, Wolfgang¹ 

[1] Institute of Molecular Biology, Vienna Biocenter, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria

关键词: Post-transcriptional control;    Thymidine kinase;    Inducible gene expression;    Cell cycle;    Elutriation centrifugation;   

DOI  :  10.1016/0014-5793(94)00451-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In vertebrates, endogenous thymidine kinase (TK) gene expression is strictly growth-dependent. Here we report that in continuously cycling Ltk⁻ mouse fibroblasts, stably transfected with a vector expressing human TK cDNA from a constitutive promoter, enzyme activity rises 8-fold at the G₁/S phase transition and declines again in G₂. The mechanism did not involve changes in protein stability. When hTK was put under the control of a hormone-inducible promoter, production of high mRNA levels following addition of dexamethasone did not result in any enzyme activity in resting NIH-3T3tk⁻ cells. After growth stimulation with serum, TK activity rose together with the onset of DNA synthesis only in the simultaneous presence of the hormone.

【 授权许可】

Unknown