Protein kinase C inhibitors induce apoptosis in human malignant glioma cell lines

FEBS Letters

Couldwell, William T.²  Law, Ronald E.²  Hinton, David R.²  Sebat, Ibrahim²  Weiss, Martin H.²  Chen, Thomas C.²  He, Shikun¹ 

[1] Departments of Pathology, The University of Southern California School of Medicine, Los Angeles, CA 90033, USA;Departments of Neurological Surgery, The University of Southern California School of Medicine, Los Angeles, CA 90033, USA

关键词: Protein kinase C;    Apoptosis;    Glioma;    Staurosporine;    Tamoxifen;    PKC;    protein kinase C;    ATCC;    American Type Culture Collection;    FCS;    fetal calf serum;    MEM;    minimal essential medium;    PBS;    phosphate-buffered saline;    TNF-α;    tumor necrosis factor-alpha;    PMA;    4-phorbol-12-myristate-13 acetate;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/0014-5793(94)00415-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previous work has demonstrated the importance of the protein kinase C (PKC) system in regulating glioma growth, and has led to clinical trials utilizing PKC inhibitors as adjuncts in the therapy of patients harboring malignant gliomas. This study was performed to explore the possibility that inhibition of PKC in gliomas was triggering an apoptosis signal. Glioma cell lines were treated with PKC inhibitors staurosporine (10 nM), and tamoxifen (10 μM). DNA from cells treated with each of these drugs exhibited a ‘ladder’ pattern of oligonucleosome-sized fragments characteristic of apoptosis, thus suggesting that in glioma cells, these drugs may be cytocidal in action.

【 授权许可】

Unknown