Development and characterization of a protein kinase C β‐isozyme‐deficient T‐cell line

FEBS Letters

Long, Aideen¹  Kelleher, Dermot¹ 

[1] Department of Clinical Medicine, Trinity College Dublin, Dublin 8, Ireland

关键词: Protein kinase C;    T lymphocyte;    Interleukin 2;    Staurosporine;    H-7;    Differentiation;    Proliferation;   

DOI  :  10.1016/0014-5793(92)80264-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the human T-cell lymphoma line, HuT 78, proliferation and phorbol ester-induced growth arrest and differentiation were inhibited by the protein kinase C (PKC) inhibitor, staurosporine. By contrast, an alternative PKC inhibitor, H-7, inhibited proliferation but not phorbol ester-induced growth arrest. The cell line was found to contain both α and β isoforms of PKC by Western blot techniques. A cell line, K-4, was cloned from HuT 78 in the presence of H-7 and this clone was found to be positive for PKC-α only. PKC-β did not return on cultivation in the absence of H-7. Proliferation of K-4 was insensitive to inhibition with both H-7 and staurosporine. However, phorbol ester-induced growth arrest remained staurosporine sensitive. Phorbol-stimulated IL-2 secretion was minimal in the PKC-β-deficient cell line. These data suggest that PKC-β may be a regulatory enzyme for proliferation and stimulated interleukin-2-secretion in HuT 78 cells. Heterogeneity of responses to PKC activation may reflect the use of different isozymes in different intracellular pathways. The K-4 cell line should provide a useful tool in the dissection of involvement of PKC isozymes in cellular function.

【 授权许可】

Unknown