Inhibition of carnitine palmitoyltransferase I by hepatocyte swelling

FEBS Letters

Guzmán, Manuel¹  Zammit, Victor A.²  Castro, José¹  Velasco, Guillermo¹ 

[1] Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Complutense University, 28040-Madrid, Spain;Hannah Research Institute, Ayr, KA6 5HL, Scotland, UK

关键词: Carnitine palmitoyltransferase I;    Hepatocyte swelling;    Glutamine;    Ketogenesis;   

DOI  :  10.1016/0014-5793(94)00405-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of hepatocytes under conditions known to increase their volume, i.e. with amino acids (glutamine, proline) or in hypo-osmotic medium, decreased carnitine palmitoyl-transferase I (CPT-I) activity. This effect of hepatocyte swelling was antagonized by okadaic acid and dibutyryl-cAMP. Physiological concentrations of glutamate inhibited CPT-I activity in digitonin-permeabilized hepatocytes but not in isolated mitochondria. Results suggest that the amino acid-induced inhibition of CPT-I shares a common mechanism with the amino acid-induced stimulation of acetyl-CoA carboxylase and glycogen synthase [(1993) Eur. J. Biochem. 217, 1083–1089].

【 授权许可】

Unknown