The source of malonyl‐CoA in rat heart

FEBS Letters

Dubelaar, Marie-Louise¹  Luyt-Houwen, Inez E.M.¹  Scholte, Hans R.¹  Huismann, Willem C.¹ 

[1] Department of Biochemistry I, Erasmus University, PO Box 1738, 3000 DR Rotterdam, The Netherlands

关键词: Acetyl-CoA carboxylase;    Calcium paradox;    Carnitine palmitoyltransferase I;    Malonyl-CoA Propionyl-CoA carboxylase;    (Rat heart);   

DOI  :  10.1016/0014-5793(86)81182-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The formation of malonyl-CoA in rat heart is catalyzed by cytosolic acetyl-CoA carboxylase. The existence of this enzyme in heart is difficult to prove by the abundant occurrence of mitochondrial propionyl-CoA carboxylase, which is also able to catalyze the carboxylation of acetyl-CoA. We used the calcium paradox as a tool to separate cytosolic components from the remaining heart, and found that acetyl-CoA carboxylase activity was preferentially released, like lactate dehydrogenase and carnitine, while propionyl-CoA carboxylase was almost fully retained. Acetyl-CoA carboxylase activity was determined after activation by citrate ion and Mg²⁺. The activity decreased to 64% by 48 h of fasting.

【 授权许可】

Unknown