Structural features of the gene encoding human muscle type carnitine palmitoyltransferase I

FEBS Letters

Hashimoto, Yoshiko²  Shinohara, Yasuo²  Terada, Hiroshi²  Shima, Atsushi¹  Yamanaka, Yasuhisa²  Yamazaki, Naoshi² 

[1] Nutrition Research Institute, Otsuka Pharmaceutical Factory, Inc., Naruto 772, Japan;Faculty of Pharmaceutical Sciences, University of Tokushima, Shomachi-1, Tokushima 770, Japan

关键词: Carnitine palmitoyltransferase I;    Gene structure;    Carnitine system;    CPTI;    carnitine palmitoyltransferase I;    CPTI-M;    muscle-type carnitine palmitoyltransferase I;    CPTI-L;    liver-type carnitine palmitoyltransferase I;    CPTII;    carnitine palmitoyltransferase II;    RT-PCR;    reverse transcription followed by the polymerase chain reaction;    RACE;    rapid amplification of cDNA ends;    bp;    base pair(s);   

DOI  :  10.1016/S0014-5793(97)00561-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated a human muscle type of carnitine palmitoyltransferase I (CPTI-M) genomic clone and determined its entire nucleotide sequence. By comparison of the nucleotide sequence of the genomic clone with that of cDNA, we determined the intron/exon junctions. For detection of the exon(s) in the 5′-region of the CPTI-M gene, we isolated cDNA clones corresponding to the 5′-region of its transcript by 5′-rapid amplification of cDNA ends (5′-RACE method). Results showed two alternative exons, 1A and 1B, that do not encode amino acids in the 5′-region of the human CPTI-M gene. The gene encoding human CPTI-M was found to consist of two 5′-non-coding exons, 18 coding exons and one 3′-non-coding exon spanning approximately 10 kbp. Furthermore, on analysis of the 5′-flanking region, a putative gene encoding a ‘choline kinase homologue’ was found to be located only about 300 bp upstream from exon 1A of the human CPTI-M gene. Comparison of the gene structure of human CPTI-M with the reported partial gene structure of human liver type CPTI (CPTI-L) showed that the intron insertion sites were completely conserved in these two genes.

【 授权许可】

Unknown