Nucleic acid‐binding regions of the second‐largest subunit of Drosophila RNA polymerase II identified by Southwestern blotting

FEBS Letters

Bautz, Ekkehard K.F.¹  Kontermann, Roland E.¹ 

[1] Institute of Molecular Genetics, University of Heidelberg, Im Neuenheimer Feld 230, D-69120 Heidelberg, Germany

关键词: Non-specific DNA binding;    RNA polymerase;    Fusion protein;    Southwestern blotting;    Transcription;   

DOI  :  10.1016/0014-5793(94)00380-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Analysing overlapping bacterially expressed fragments of the second-largest subunit of Drosophila melanogaster RNA polymerase II in Southwestern DNA binding assays we have identified regions that have the potential to bind nucleic acids non-specifically. A region exhibiting strong DNA binding is located in the N-terminal part of the molecule (amino acids 357–504) and some weak DNA binding is observed for the C-terminal part (amino acids 860–1160). The non-specific DNA binding behavior of these regions is similar to that of the native enzyme. Most of the known mutations responsible for rifampicin resistance map to a region of the Escherichia coli β subunit corresponding to the N-terminal nucleic acid-binding region, indirectly supporting the notion that this region participates in interaction with the RNA transcript in ternary complexes.

【 授权许可】

Unknown