The aromatic nature of residue 66 of the 11‐kDa subunit of ubiquinol‐cytochrome c oxidoreductase of the yeast Saccharomyces cerevisiae is important for the assembly of a functional enzyme

FEBS Letters

Grivell, Leslie A.¹  Hemrika, Wieger²  Berden, Jan A.²  Lobo-Hajdu, Gisele² 

[1] Section for Molecular Biology, Department of Molecular Cell Biology, University of Amsterdam, Amsterdam, The Netherlands;E.C. Slater Institute, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands

关键词: Ubiquinol-cytochrome c oxidoreductase;    Subunit;    Mutagenesis;    Assembly;    Saccharomyces cerevisiae;    Q2H2;    2;    3-dimethoxy-5-methyl-6-geranyl-1;    4-benzoquinol;   

DOI  :  10.1016/0014-5793(94)00346-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transformation of multi- and single-copy plasmids carrying a mutated version (LTN2, region 66-YWYWW-70 replaced by SASAA) of QCR8, the gene encoding the 11-kDa subunit of ubiquinol-cytochrome c oxidoreductase of Saccharomyces cerevisiae, to a QCR8⁰ strain indicated the importance of this aromatic region for the assembly of a functional enzyme. Sequencing of plasmids giving spontaneous restoration of growth to some colonies among the single-copy LTN2 transformants showed that changing the sequence SASAA into the sequence FASAA could, to a large extent, overcome the observed assembly defect, indicating the importance of the aromatic nature of residue 66.

【 授权许可】

Unknown